| Expression pattern: |
UP |
| Associated gene: |
JAK2, STAT6, ESRP1, Ago2, MUC5AC, SPDEF, FOXA2 |
| Associated microRNA: |
miR-452-5p, miR-23b-3p, miR-34b-5p, miR-182-5p |
| Biological function: |
Promotes goblet cell metaplasia, mucous hypersecretion, airway hyperresponsiveness, and allergic airway epithelial remodeling; knockdown attenuates OVA-induced inflammatory infiltration, PAS staining, MUC5AC and SPDEF expression, and airway hyperresponsiveness. |
| Molecular mechanism: |
circZNF652 acts as a ceRNA sponge for miR-452-5p, relieving miR-452-5p-mediated suppression of JAK2 and activating JAK2/STAT6 signaling; ESRP1 binds flanking introns and promotes circZNF652 biogenesis. |
| Biological pathway or process: |
JAK/STAT (promotes); ceRNA regulation (promotes); proliferation (other); apoptosis (other); goblet cell metaplasia (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; RNA-seq; Transfection; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Potential therapeutic target for allergic airway inflammation and asthmatic airway remodeling; blockage of circZNF652 may provide an alternative therapeutic intervention approach. |
| Description: |
circZNF652 is up-regulated in airway epithelia from children with asthma and OVA-induced allergic airway inflammation mouse models. It promotes goblet cell metaplasia and airway remodeling by sponging miR-452-5p, thereby increasing JAK2 expression and activating JAK2/STAT6 signaling; ESRP1 promotes its biogenesis by binding flanking intronic motifs. |
| Confidence score: |
0.8398 |