| Expression pattern: |
UP |
| Associated gene: |
GOT2, Ago2 |
| Associated microRNA: |
miR-1298-5p |
| Biological function: |
promotes proliferation, cell cycle progression, migration, invasion, angiogenesis and tumor growth; inhibits apoptosis and autophagy in NSCLC cells |
| Molecular mechanism: |
ceRNA mechanism: circ_0003028 sponges miR-1298-5p to regulate GOT2 expression |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); angiogenesis (promotes); autophagy (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ_0003028 is up-regulated in NSCLC tissues and cell lines and mainly localizes to the cytoplasm. It promotes malignant phenotypes (proliferation, migration/invasion, angiogenesis) and tumor growth by sponging miR-1298-5p to increase GOT2 expression, while its knockdown increases apoptosis and autophagy. |
| Confidence score: |
0.8201 |