circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBM tissues; normal adjacent tissues
Cell lines:

U539; U251
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: YAP1, VAP1, PI3K, AKT, MMP-9, cleaved-Caspase-3, cleaved-Caspase-9, N-cadherin, E-cadherin, Vimentin, Zeb1
Associated microRNA: miR-199a-5p
Biological function: CircPVT1 promotes GBM cell viability, migration and EGF-induced EMT, and inhibits apoptosis; silencing circPVT1 suppresses viability, migration and EMT and promotes apoptosis in U539 and U251 cells.
Molecular mechanism: Silencing circPVT1 up-regulates miR-199a-5p, thereby inhibiting GBM cell viability, migration and EGF-induced EMT, promoting apoptosis, and repressing YAP1/VAP1 and PI3K/AKT pathways.
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); EMT (promotes); PI3K/AKT (promotes); Hippo/YAP (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot
Clinical significance:

CircPVT1 may provide an advanced target for clinical therapy in GBM.
Description:

hsa_circ_PVT1/circPVT1 is up-regulated in GBM tissues. In GBM cell lines, circPVT1 knockdown suppresses viability, migration, EGF-induced EMT and PI3K/AKT/YAP1-related signaling while promoting apoptosis, largely through up-regulation of miR-199a-5p. The study suggests circPVT1 as a potential therapeutic target for GBM.
Confidence score:

0.5414
Other information
Title:

Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p.

Journal: Artificial cells, nanomedicine, and biotechnology
Published: 2020
PubMed ID: 31865777
Study type:

combined biological and clinical study
Data availability: -
Code availability: -