| Expression pattern: |
UP |
| Associated gene: |
WNT3, VEGFA |
| Associated microRNA: |
miR-613 |
| Biological function: |
circCCT3 promotes CRC cell invasion and metastasis and inhibits apoptosis; circCCT3 knockdown suppresses invasion and induces apoptosis of CRC cells. |
| Molecular mechanism: |
circCCT3 functions as a ceRNA/sponges miR-613 to regulate WNT3 and VEGFA expression and Wnt signaling in CRC cells. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
S
|
| Validation methods: |
RT-qPCR; RNA-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; IF (Immunofluorescence); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCCT3 expression is associated with advanced CRC stage and poorer disease-free survival; low circCCT3 level is associated with higher disease-free survival. |
| Description: |
circCCT3 is up-regulated in human CRC tumors and is associated with advanced stage and poorer disease-free survival. Functionally, circCCT3 promotes CRC cell invasion/metastasis and suppresses apoptosis. Mechanistically, it sponges miR-613 to regulate WNT3, VEGFA and Wnt signaling. |
| Confidence score: |
0.7718 |