| Expression pattern: |
UP |
| Associated gene: |
TGFbeta1, p-Smad2/3, AR, ADAR2 |
| Associated microRNA: |
miRNA-1275, miRNA-646 |
| Biological function: |
Promotes prostate cancer cell invasion by inducing TGFbeta1/p-Smad2/3 signaling; knockdown of circRNA-51217 reverses R-2HG-induced invasion and signaling. |
| Molecular mechanism: |
R-2HG induces circRNA-51217 generation, partly associated with increased m6A level of TGFbeta1 mRNA; circRNA-51217 acts as a miRNA sponge for miRNA-646, relieving repression of TGFbeta1 and activating TGFbeta1/p-Smad2/3 signaling. AR suppresses this axis by regulating TGFbeta1 transcription and ADAR2-mediated circRNA generation. |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); invasion (promotes); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; ChIP / ChIP-seq; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circRNA-51217 is induced by R-2HG/IDH1 R132H-related conditions in prostate cancer cells with negative or low AR expression. It promotes PCa cell invasion by sponging miRNA-646, increasing TGFbeta1 expression, and activating TGFbeta1/p-Smad2/3 signaling; AR counteracts this pathway partly through TGFbeta1 transcriptional repression and ADAR2-mediated suppression of circRNA generation. |
| Confidence score: |
0.6596 |