circRNA basic information
circBase ID: hsa_circ_0085616
Name: hsa_circ_ASAP1
Synonym: circASAP1
Host Gene: ASAP1
Genomic location(hg19): chr8:131370262-131374017:-
Genomic location(hg38): chr8:130358016-130361771:-
Subcellular localization: cytoplasm; exosome; extracellular
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary HCC tissues; HCC tumor tissues; plasma; exosomes from plasma; paraffin-embedded tissue samples
Cell lines:

MHCC97L; MHCC97H; HCCLM3; L0-2; HepG2; PLC/PRF/5; Huh7
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK1, CSF-1, AGO2
Associated microRNA: miR-326, miR-532-5p
Biological function: Promotes HCC cell proliferation, colony formation, migration, invasion, tumor growth, pulmonary metastasis, and tumor-associated macrophage infiltration.
Molecular mechanism: circASAP1 acts as a ceRNA sponge for miR-326 and miR-532-5p, thereby regulating MAPK1 and CSF-1 expression, ERK1/2 signaling, and CSF-1-mediated tumor-associated macrophage infiltration.
Biological pathway or process:

ceRNA regulation (promotes); ERK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); immune regulation (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ELISA; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circASAP1 expression is associated with HCC pulmonary metastasis/recurrence, poorer overall survival, higher cumulative recurrence, and prognostic prediction in patients with HCC.
Description:

circASAP1 is an up-regulated human circRNA in metastatic/recurrent HCC and is associated with pulmonary metastasis and poor prognosis. It promotes HCC proliferation, invasion, tumor growth, and lung metastasis by sponging miR-326 and miR-532-5p, thereby increasing MAPK1 and CSF-1 signaling and enhancing tumor-associated macrophage infiltration.
Confidence score:

0.8913
Other information
Title:

Circular RNA Sequencing Identifies CircASAP1 as a Key Regulator in Hepatocellular Carcinoma Metastasis.

Journal: Hepatology (Baltimore, Md.)
Published: 2020
PubMed ID: 31838741
Study type:

combined biological and clinical study
Data availability: -
Code availability: -