| Expression pattern: |
UP |
| Associated gene: |
BTBD7, ROCK2, MMP-2, MMP-9 |
| Associated microRNA: |
miR-936 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion, and tumorigenicity |
| Molecular mechanism: |
ceRNA mechanism: circZCCHC2 sponges miR-936 to upregulate BTBD7 and activate Rho/ROCK2 signaling |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; Clinical Sample Validation; Survival Analysis; In Vivo Animal Model |
| Clinical significance: |
High circZCCHC2 expression indicates an unfavorable prognosis and predicts shorter overall survival in HCC patients. |
| Description: |
circZCCHC2 is up-regulated in HCC tissues and cell lines and its high expression predicts worse overall survival. Functionally, circZCCHC2 promotes proliferation and metastatic behaviors and enhances xenograft tumor growth. Mechanistically, it sponges miR-936 to increase BTBD7 and activate the Rho/ROCK2 pathway (with downstream changes such as ROCK2, MMP-2 and MMP-9). |
| Confidence score: |
0.866 |