| Expression pattern: |
UP |
| Associated gene: |
TBX2, AGO2, CYP24A1 |
| Associated microRNA: |
miR-331-3p |
| Biological function: |
Promotes CRC cell proliferation, autophagy, migration and invasion, inhibits apoptosis and necrosis, and promotes tumor growth in vivo. |
| Molecular mechanism: |
ceRNA mechanism: circ_0060927 sponges miR-331-3p to upregulate TBX2 expression. |
| Biological pathway or process: |
proliferation (promotes); autophagy (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0060927 may be a biomarker for CRC; it could distinguish CRC tissue from normal tissue with an AUC value of 0.8697, and expression was higher in CRC tissues from patients with TNM III than TNM II and TNM I. |
| Description: |
circ_0060927 is up-regulated in colorectal cancer tissues and cell lines and may serve as a diagnostic biomarker. Functionally, it promotes CRC progression by enhancing proliferation, autophagy, migration, invasion and tumor growth while suppressing apoptosis and necrosis. Mechanistically, circ_0060927 acts as a ceRNA by sponging miR-331-3p, thereby upregulating TBX2. |
| Confidence score: |
0.8489 |