| Expression pattern: |
DN |
| Associated gene: |
ITM2A, PD-L1, AGO2 |
| Associated microRNA: |
hsa-miR-526b-5p, hsa-miR-583 |
| Biological function: |
Suppresses LUSC cell proliferation, migration, and invasion; promotes apoptosis; enhances antitumor immune responses by promoting PD-L1 degradation. |
| Molecular mechanism: |
Acts as a ceRNA sponge for hsa-miR-526b-5p to upregulate ITM2A, activating autophagy and promoting PD-L1 degradation, enhancing CD8+ T-cell cytotoxicity. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); autophagy (promotes); immune regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Transfection; Colony Formation Assay; Transwell Assay; TUNEL; In Vivo Animal Model; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis; RNA-seq; IF (Immunofluorescence) |
| Clinical significance: |
circITFG2 down-regulation profile negatively correlates with tumor progression and may be a prognostic biomarker/diagnostic marker for LUSC; higher circITFG2 expression correlates with earlier LUSC T stages. |
| Description: |
circITFG2 is down-regulated in LUSC and functions as a tumor suppressor. It sponges hsa-miR-526b-5p to upregulate ITM2A, promoting autophagy-dependent PD-L1 degradation and enhancing antitumor immune activity while inhibiting proliferation, migration, and invasion. |
| Confidence score: |
0.867 |