| Expression pattern: |
UP |
| Associated gene: |
Smad3 |
| Associated microRNA: |
miR-617 |
| Biological function: |
promotes osteogenesis of DPSCs |
| Molecular mechanism: |
ceRNA (miRNA sponge) mechanism: circSIPA1L1 sponges miR-617 to regulate Smad3 (phosphorylated Smad3) |
| Biological pathway or process: |
ceRNA regulation (promotes); TGF-beta/SMAD (promotes); osteogenesis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); CCK8; EdU Staining; Cell Cycle Assay; ALP staining; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
potential therapeutic strategies for the bone regeneration through targeting osteogenesis of DPSCs |
| Description: |
circSIPA1L1 is upregulated during osteogenic differentiation of dental pulp stem cells and promotes osteogenesis. Mechanistically, it acts as a miRNA sponge for miR-617 in the cytoplasm, relieving repression on Smad3 (including phosphorylated Smad3) and enhancing osteogenic marker expression and mineralization, supported by in vitro assays and a nude mouse subcutaneous implantation model. |
| Confidence score: |
0.7263 |