| Expression pattern: |
UP |
| Associated gene: |
ASCT2 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis; promotes intracellular glutamine metabolism |
| Molecular mechanism: |
circCPA4 acts as a miRNA sponge for miR-145-5p to upregulate ASCT2 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circCPA4 expression was significantly associated with tumor size, tumor node metastasis (TNM) stage and lymph node metastasis of NSCLC patients |
| Description: |
circCPA4 is up-regulated in NSCLC tissues/cells and promotes malignant phenotypes (proliferation, migration, invasion) while inhibiting apoptosis. Mechanistically, circCPA4 sponges miR-145-5p to increase ASCT2 expression, enhancing intracellular glutamine/glutamate/alpha-KG levels and supporting glutamine metabolism; circCPA4 knockdown suppresses tumor growth in a xenograft model. |
| Confidence score: |
0.8172 |