circRNA basic information
circBase ID: hsa_circ_0000285
Name: hsa_circ_HIPK3
Synonym: circ-0000285
Host Gene: HIPK3
Genomic location(hg19): chr11:33362513-33363232:+
Genomic location(hg38): chr11:33340967-33341686:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenograft tumor; lung
Cell lines:

hFOB1.19; Saos2; MG63; U2OS; SJSA1; HOS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TGFB2, AGO2
Associated microRNA: hsa-miRNA-599, miRNA-599
Biological function: promotes osteosarcoma cell proliferation, migration, tumor growth, and metastasis
Molecular mechanism: circ-0000285 functions as a ceRNA by sponging miRNA-599 and positively regulating TGFB2 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Sanger Sequencing; RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

providing a novel direction for clinical diagnosis and treatment of osteosarcoma
Description:

Hsa_circ_0000285/circ-0000285 is up-regulated in osteosarcoma cell lines and promotes osteosarcoma proliferation, migration, tumor growth, and metastatic nodule formation. Mechanistically, it is mainly cytoplasmic and functions as a ceRNA that sponges miRNA-599, thereby positively regulating TGFB2 expression through the circ-0000285/miRNA-599/TGFB2 axis.
Confidence score:

0.8207
Other information
Title:

Hsa_circ_0000285 functions as a competitive endogenous RNA to promote osteosarcoma progression by sponging hsa-miRNA-599.

Journal: Gene therapy
Published: 2020
PubMed ID: 31784675
Study type:

biological research
Data availability: GSE96964; available from the corresponding author on reasonable request
Code availability: -