circRNA basic information
circBase ID: hsa_circ_0003441
Name: hsa_circ_TDRD3
Synonym: circTDRD3
Host Gene: TDRD3
Genomic location(hg19): chr13:61013821-61041513:+
Genomic location(hg38): chr13:60439687-60467379:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissues; adjacent benign tissue; xenograft tumor tissues; lung tissues
Cell lines:

HGC27; MGC803; MKN45; MKN28; SGC7901; AGS; GES-1; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ITGA2, AKT, p-AKT, Foxo1, p-Foxo1, ATF4, Cyclin D1, TDRD3
Associated microRNA: miR-891b
Biological function: Promotes gastric cancer cell proliferation, cell cycle progression, migration, invasion, tumor growth and lung metastasis.
Molecular mechanism: circTDRD3 acts as a miR-891b sponge to upregulate ITGA2, activate AKT signaling, and form an ATF4-mediated positive feedback loop involving TDRD3/circTDRD3 transcriptional regulation.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); cell cycle (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circTDRD3 expression is positively correlated with tumor size and lymph node metastasis, indicates poor prognosis, and may serve as a biomarker for early diagnosis and prognostic assessment.
Description:

circTDRD3 (hsa_circ_0003441), derived from TDRD3, is up-regulated in gastric cancer tissues and cell lines and is associated with tumor size, lymph node metastasis, and poor prognosis. It promotes gastric cancer proliferation, migration, invasion, tumor growth, and lung metastasis by sponging miR-891b to increase ITGA2 expression and activate AKT signaling, while ATF4 further promotes TDRD3/circTDRD3 transcription in a positive feedback loop.
Confidence score:

0.8981
Other information
Title:

ATF4-mediated circTDRD3 promotes gastric cancer cell proliferation and metastasis by regulating the miR-891b/ITGA2 axis and AKT signaling pathway.

Journal: Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
Published: 2023
PubMed ID: 37062785
Study type:

combined biological and clinical study
Data availability: The datasets in this study are available from the corresponding author on reasonable request.
Code availability: -