| Expression pattern: |
UP |
| Associated gene: |
YB-1, Vimentin, N-cadherin, E-cadherin |
| Associated microRNA: |
miR-382-5p |
| Biological function: |
Promotes proliferation, migration, invasion and metastasis of osteosarcoma cells, impedes apoptosis, and is associated with early relapsing in OS patients. |
| Molecular mechanism: |
Circ_0001658 acts as a miR-382-5p sponge and positively regulates YB-1 expression, thereby promoting malignant behaviors of OS cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0001658 in tumour tissues was closely correlated with early relapsing of OS patients. |
| Description: |
Circ_0001658 is up-regulated in osteosarcoma tissues and cell lines. It promotes OS cell proliferation, migration, invasion and metastasis while suppressing apoptosis through a miR-382-5p/YB-1 regulatory axis, and its high expression is associated with early relapse in OS patients. |
| Confidence score: |
0.6536 |