| Expression pattern: |
DN |
| Associated gene: |
ZEB1, ZEB1 mRNA |
| Associated microRNA: |
hsa-mir200a-3p, hsa-mir141-3p, mir200a |
| Biological function: |
circZEB1 participates in the ZEB1-mir200 ceRNA circuit related to melanoma aggressiveness and phenotypic switching; it is reduced in metastatic melanoma cell lines compared with matched primary melanoma cell lines, but remains high and relatively constant during CSC-marker-associated phenotypic switching of IgR39 cells. |
| Molecular mechanism: |
ceRNA mechanism involving mir200-family miRNA binding and competition with ZEB1 mRNA; a mathematical model suggests that constant high circZEB1 during phenotypic switching can be explained by a limiting back-splicing factor. |
| Biological pathway or process: |
ceRNA regulation (promotes); EMT (other); stemness (other); metastasis (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
This study investigated circZEB1 in human melanoma cells as part of the ZEB1-mir200 ceRNA circuit related to EMT, aggressiveness, and phenotypic switching. circZEB1 was reduced in metastatic melanoma cell lines relative to matched primary melanoma cell lines, but remained high and relatively constant during CSC-associated phenotypic switching. The authors propose that its constant expression can be explained by a limiting back-splicing factor under high ZEB1 transcription conditions. |
| Confidence score: |
0.4375 |