| Expression pattern: |
UP |
| Associated gene: |
GAB1 |
| Associated microRNA: |
miR-520a-3p |
| Biological function: |
Circ_CDK14 promotes OS cell proliferation, metastasis, tumorigenesis and tumor growth, and suppresses apoptosis; circ_CDK14 knockdown inhibits proliferation, migration, invasion and in vivo tumor growth while promoting apoptosis and cell cycle arrest. |
| Molecular mechanism: |
Circ_CDK14 functions as a ceRNA by sponging miR-520a-3p, thereby modulating GAB1 expression through the miR-520a-3p/GAB1 axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
Circ_CDK14 was highly expressed in OS tissues and may be a novel potential therapeutic target for OS. |
| Description: |
circ_CDK14/hsa_circ_0001721 is up-regulated in osteosarcoma tissues and cell lines. It promotes OS progression by acting as a miR-520a-3p sponge and regulating the downstream target GAB1; knockdown of circ_CDK14 inhibits proliferation, migration, invasion and xenograft tumor growth while inducing apoptosis. |
| Confidence score: |
0.7421 |