circRNA basic information
circBase ID: hsa_circ_0084615
Name: hsa_circ_ASPH
Synonym: circ_0084615
Host Gene: ASPH
Genomic location(hg19): chr8:62593526-62596747:-
Genomic location(hg38): chr8:61680967-61684188:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; nearby non-tumor tissues

Cell lines:

FHC; SW620; DLD1; SW480; HT-29; HUVECs

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: ONECUT2, EIF4A3
Associated microRNA: miR-599
Biological function: promotes CRC cell proliferation, migration, invasion and angiogenesis; promotes tumorigenesis in vivo
Molecular mechanism: ceRNA mechanism: circ_0084615 sponges miR-599 to upregulate ONECUT2; EIF4A3 induces circ_0084615 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); EMT (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model

Clinical significance:

Elevated circ_0084615 in CRC tissues is linked to TNM stages, lymph node metastasis, differentiation and overall survival; high circ_0084615 correlates with poor overall survival.

Description:

circ_0084615 (ASPH-derived) is up-regulated in colorectal cancer and functions as an oncogenic circRNA. It promotes proliferation, migration/invasion, EMT-related changes and angiogenesis, at least partly by sponging miR-599 to increase ONECUT2, and its biogenesis is positively regulated by the RBP EIF4A3.

Confidence score:

0.873

Other information
Title:

EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2021
PubMed ID: 34253241
Study type:

combined biological and clinical study

Data availability: -
Code availability: -