| Expression pattern: |
UP |
| Associated gene: |
LY6E, AGO2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
Promotes migration, invasion, proliferation, tumor growth and metastasis of cholangiocarcinoma cells; inhibits apoptosis; exosomal circ-0000284 stimulates migration and proliferation of surrounding normal cells and suppresses their apoptosis. |
| Molecular mechanism: |
circ-0000284 acts as a ceRNA by sponging miR-637 to up-regulate LY6E expression; circ-0000284 can also be transferred via exosomes from cholangiocarcinoma cells to surrounding normal cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; Transfection; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); FISH / smFISH; Bioinformatics Analysis; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
High expression of circ-0000284 in tumor tissues was associated with grade of differentiation, TNM stage and lymph node invasion; circ-0000284 was elevated in plasma exosomes of cholangiocarcinoma patients compared with healthy controls. |
| Description: |
circ-0000284 is up-regulated in cholangiocarcinoma cell lines, tumor tissues and plasma exosomes. It promotes cholangiocarcinoma cell proliferation, migration, invasion, tumor growth and metastasis while inhibiting apoptosis through the miR-637/LY6E ceRNA axis. Exosome-transmitted circ-0000284 can also be delivered to surrounding normal cells and promote malignant-like cellular behaviors. |
| Confidence score: |
0.8511 |