circRNA basic information
circBase ID: -
Name: hsa_circ_FOXP1
Synonym: circ-FOXP1
Host Gene: FOXP1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; serum
Cell lines:

SNU-387; HepG2; Hep3B; Huh7; SMMC-7721; HCCLM3; LO2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SOX9, EGFR, cyclin D1, CD81, PI4KA, CDK6, MDM2, E2F3, PGK1
Associated microRNA: miR-875-3p, miR-421
Biological function: Promotes HCC cell proliferation, invasion, tumor growth and aggressive progression; inhibits apoptosis; acts as a diagnostic and prognostic biomarker.
Molecular mechanism: SOX9 transcriptionally activates circ-FOXP1; cytoplasmic circ-FOXP1 acts as a ceRNA by sponging miR-875-3p and miR-421, thereby increasing their target oncogenes including SOX9 and forming a positive regulatory loop.
Biological pathway or process:

proliferation (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; ChIP / ChIP-seq; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

High circ-FOXP1 correlated with larger tumor size, microvascular invasion, advanced TNM stage and poor prognosis; serum circ-FOXP1 discriminated HCC patients from healthy controls with AUC 0.9318.
Description:

circ-FOXP1 is up-regulated in HCC tissues, serum and cell lines and functions as an oncogenic circRNA. SOX9 induces circ-FOXP1 expression, while cytoplasmic circ-FOXP1 sponges miR-875-3p and miR-421 to elevate oncogenic targets including SOX9, promoting proliferation, invasion, tumor growth and poor prognosis. Serum circ-FOXP1 also shows diagnostic potential for distinguishing HCC patients from healthy controls.
Confidence score:

0.8413
Other information
Title:

Circular RNA circ-FOXP1 induced by SOX9 promotes hepatocellular carcinoma progression via sponging miR-875-3p and miR-421.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Published: 2020
PubMed ID: 31698267
Study type:

combined biological and clinical study
Data availability: GSE128274; GSE94508; GSE97332; Supplementary material at doi:https://doi.org/10.1016/j.biopha.2019.109517
Code availability: -