| Expression pattern: |
UP |
| Associated gene: |
AMPK, PI3K, AKT, mTOR, Bcl-2, Bax, cleaved-caspase-3, MMP-2, MMP-9 |
| Associated microRNA: |
miR-505 |
| Biological function: |
circNRIP1 exerts oncogenic roles in renal carcinoma cells; circNRIP1 knockdown inhibits cell viability, colony formation, and migration, and promotes apoptosis in ACHN and CAKI-1 cells. |
| Molecular mechanism: |
circNRIP1 negatively regulates miR-505 and activates AMPK and PI3K/AKT/mTOR signaling cascades; si-circNRIP1 deactivates these pathways by upregulating miR-505. |
| Biological pathway or process: |
AMPK (promotes); PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot |
| Clinical significance: |
circNRIP1 was highly expressed in renal tumor tissues compared with nontumor controls, suggesting association with renal cancer development. |
| Description: |
circNRIP1 is up-regulated in renal tumor tissues and functions as an oncogenic circRNA in ACHN and CAKI-1 renal carcinoma cells. Knockdown of circNRIP1 suppresses cell viability, colony formation, migration, and AMPK/PI3K/AKT/mTOR signaling while promoting apoptosis, at least partly through upregulation of miR-505. |
| Confidence score: |
0.5434 |