| Expression pattern: |
DN |
| Associated gene: |
E-cadherin, vimentin |
| Associated microRNA: |
hsa-miR-149-5p, hsa-miR-136-5p, hsa-miR-155-5p |
| Biological function: |
Reduced hsa_circ_0004491 expression is associated with lymph node metastasis and promotes OSCC cell migration, invasion, and EMT; overexpression suppresses migration, invasion, and EMT-related changes. |
| Molecular mechanism: |
hsa_circ_0004491 may function through circRNA-miRNA interaction, including predicted binding to hsa-miR-136-5p, hsa-miR-149-5p and hsa-miR-155-5p; hsa-miR-155-5p increased after hsa_circ_0004491 knockdown, and EMT markers E-cadherin and vimentin were altered. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0004491 expression was negatively associated with lymph node metastasis and had diagnostic value as an indicator of OSCC, with ROC AUC of 0.7510. |
| Description: |
hsa_circ_0004491 is a human ORC4-derived circRNA downregulated in OSCC tissues and cell lines. Low expression is associated with lymph node metastasis and loss of hsa_circ_0004491 promotes OSCC migration, invasion, and EMT-like marker changes, while overexpression suppresses these malignant phenotypes. The study suggests hsa_circ_0004491 may serve as a diagnostic/metastasis-related biomarker and may act partly through miRNA-related regulation, especially involving hsa-miR-155-5p. |
| Confidence score: |
0.7078 |