circRNA basic information
circBase ID: hsa_circ_0005185
Name: hsa_circ_PRKD1
Synonym: circ_0005185 / circ_0 005185
Host Gene: PRKD1
Genomic location(hg19): chr14:30093357-30105700:-
Genomic location(hg38): chr14:29624151-29636494:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

prostate cancer tissues; para-cancer samples; adjacent normal tissues; HSPC tumor tissue; CRPC tumor tissue; xenograft tumor
Cell lines:

LNCaP; DU145; C4-2; PC3; 22RV1; RWPE1; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: OTUB1, RAB8A, GLI3R, AR, CCND1, c-MYC, KLK3, TMPRSS2
Associated microRNA: -
Biological function: Inhibits prostate cancer proliferation and migration, mitigates enzalutamide resistance, promotes primary cilia formation, suppresses Hedgehog signaling and AR activity, and inhibits tumor growth in vivo.
Molecular mechanism: Acts as a molecular scaffold binding OTUB1 and RAB8A, enhances OTUB1-RAB8A interaction, promotes OTUB1-mediated deubiquitination (K48-linked) of RAB8A to stabilize RAB8A protein, thereby promoting ciliogenesis, increasing GLI3R, and inhibiting Hedgehog signaling and AR activity.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); Hedgehog (inhibits); drug resistance (inhibits); other pathway/process (promotes)
Detected method:
Q
H
S
M
Validation methods:

RNA-seq; Microarray; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Western Blot; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model
Clinical significance:

Low circ_0005185 expression was significantly associated with higher T stage, higher N stage, and higher Gleason Score.
Description:

circ_0005185 (hsa_circ_0005185), derived from PRKD1, is down-regulated in prostate cancer/CRPC and enzalutamide-resistant contexts. It binds OTUB1 and RAB8A as a scaffold to promote OTUB1-mediated K48 deubiquitination and stabilization of RAB8A, enhancing primary cilia formation, increasing GLI3R, inhibiting Hedgehog signaling and AR activity, and suppressing tumor progression and drug resistance.
Confidence score:

0.8785
Other information
Title:

Primary Cilia Formation Mediated by Hsa_Circ_0005185/OTUB1/RAB8A Complex Inhibits Prostate Cancer Progression by Suppressing Hedgehog Signaling Pathway.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published: 2025
PubMed ID: 39785769
Study type:

combined biological and clinical study
Data availability: GSE113153; GSE118959; TCGA
Code availability: -