| Expression pattern: |
UP |
| Associated gene: |
STAT3 |
| Associated microRNA: |
miR-296-5p |
| Biological function: |
circ_0076305 increases cisplatin resistance of NSCLC cells and is associated with DDP-resistant NSCLC tissues and cell lines. |
| Molecular mechanism: |
circ_0076305 acts as a sponge of miR-296-5p to release or positively modulate STAT3 expression, thereby promoting DDP resistance in NSCLC cells. |
| Biological pathway or process: |
drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; circRIP; Luciferase Reporter Assay; Transfection; MTT; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0076305 expression was associated with DDP resistance and lymph node metastasis in NSCLC patients, and the authors suggest it may provide insight for diagnosis and therapy of NSCLC. |
| Description: |
This study reports that circ_0076305 is up-regulated in NSCLC tissues and DDP-resistant NSCLC contexts. Functionally, circ_0076305 promotes cisplatin resistance in NSCLC cells. Mechanistically, it acts as a miR-296-5p sponge to positively regulate STAT3 expression, forming a circ_0076305/miR-296-5p/STAT3 axis related to DDP resistance. |
| Confidence score: |
0.7475 |