| Expression pattern: |
DN |
| Associated gene: |
ZEB2, NF-kB pathway, p50, p65, IkappaBalpha |
| Associated microRNA: |
miR-545 |
| Biological function: |
circPRKCI alleviates LPS-induced HK2 cell injury by promoting cell viability, inhibiting apoptosis, and reducing inflammatory cytokine production. |
| Molecular mechanism: |
circPRKCI negatively regulates miR-545, thereby upregulating the miR-545 target gene ZEB2 and inhibiting NF-kB pathway activation in LPS-induced HK2 cell injury. |
| Biological pathway or process: |
NF-kappaB (inhibits); apoptosis (inhibits); proliferation (promotes); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; ELISA; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circPRKCI was significantly down-regulated in serum samples from patients with urosepsis compared with healthy subjects, suggesting potential relevance as a disease-associated circulating marker. |
| Description: |
circPRKCI is down-regulated in serum from urosepsis patients and in LPS-treated HK2 cells. Functionally, circPRKCI overexpression protects HK2 cells from LPS-induced injury by improving viability, suppressing apoptosis, and reducing inflammatory cytokines. Mechanistically, circPRKCI acts through the miR-545/ZEB2 axis and inhibits NF-kB pathway activation. |
| Confidence score: |
0.6521 |