| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Ago2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Circ_0000514 promotes NSCLC cell growth, proliferation, cell cycle progression, migration, invasion and metastatic potential, and inhibits apoptosis. |
| Molecular mechanism: |
Circ_0000514 functions as a ceRNA/molecular sponge for miR-330-5p to positively regulate HMGA2 expression, thereby promoting NSCLC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; TUNEL; Western Blot |
| Clinical significance: |
Circ_0000514 expression was significantly associated with advanced TNM stage and linked to adverse clinicopathological indicators in NSCLC patients; the study suggests potential value for diagnosis, prognosis prediction and treatment. |
| Description: |
Circ_0000514 is an up-regulated oncogenic circRNA in NSCLC. It promotes malignant phenotypes including proliferation, cell cycle progression, migration and invasion while suppressing apoptosis, mainly through sponging miR-330-5p and up-regulating HMGA2. Its high expression is associated with advanced TNM stage and adverse clinicopathological indicators. |
| Confidence score: |
0.78 |