| Expression pattern: |
UP |
| Associated gene: |
HuR, p27 |
| Associated microRNA: |
- |
| Biological function: |
Promotes HCC growth and tumorigenesis, increases HCC cell activity and proliferation, accelerates G1 to S-phase cell cycle transition, and acts as an oncogene in HCC development. |
| Molecular mechanism: |
circBACH1 directly binds HuR and promotes HuR translocation from the nucleus to the cytoplasm, where HuR represses p27 translation; the circBACH1/HuR/p27 axis promotes HCC proliferation and cell cycle progression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RT-qPCR; RNA-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; IF (Immunofluorescence); Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circBACH1 expression is associated with poor prognosis; circBACH1 is a potential diagnostic marker, prognostic index, therapeutic target, and diagnostic biomarker in HCC. |
| Description: |
circBACH1 (hsa_circ_0061395) is upregulated in HCC tissues and cell lines, and high expression predicts poor prognosis. Functionally, it promotes HCC proliferation, cell cycle progression, and xenograft tumor growth. Mechanistically, circBACH1 binds HuR and promotes its cytoplasmic accumulation, thereby repressing p27 translation through the circBACH1/HuR/p27 axis. |
| Confidence score: |
0.8743 |