| Expression pattern: |
DN |
| Associated gene: |
CREB1, DUSP9, LDLR, PHLDA1 |
| Associated microRNA: |
- |
| Biological function: |
inhibits proliferation; inhibits migration; inhibits invasion; promotes apoptosis; blocks cell cycle in S phase; inhibits keloid progression in vivo |
| Molecular mechanism: |
hsa_circ_0026782 directly binds CREB1 (via 1-90 nt), promotes CREB1 dimerization and phosphorylation at Ser142, inhibits CREB1 binding to promoters/enhancers and suppresses CREB1-mediated transcription |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); other pathway/process (inhibits); m6A modification (other) |
| Detected method: |
Q
B
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; IF (Immunofluorescence); Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; H&E Staining |
| Clinical significance: |
providing a new target for the therapy of keloids |
| Description: |
hsa_circ_0026782 (derived from ITGA7 exon 4) is down-regulated in keloid tissues. It suppresses keloid fibroblast proliferation/migration/invasion, promotes apoptosis, and inhibits xenograft keloid growth. Mechanistically, it binds CREB1 (via the 1-90 nt region) and promotes CREB1 Ser142 phosphorylation to reduce CREB1 transcriptional activity, downregulating CREB1 target genes such as DUSP9 and LDLR. |
| Confidence score: |
0.869 |