| Expression pattern: |
UP |
| Associated gene: |
NOVA2 |
| Associated microRNA: |
miR-512-3p |
| Biological function: |
Promotes NSCLC cell proliferation, invasion and macrophage M2 polarization under hypoxia; GF suppresses these malignant phenotypes partly by decreasing circ_0059665. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-512-3p to upregulate NOVA2 in NSCLC cells under hypoxia; GF reduces circ_0059665, thereby relieving miR-512-3p suppression and decreasing NOVA2. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); macrophage polarization (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; Luciferase Reporter Assay; Western Blot; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Microarray |
| Clinical significance: |
- |
| Description: |
circ_0059665 is up-regulated in NSCLC tissues/cells and further induced by hypoxia. It promotes NSCLC proliferation, invasion, and M2 macrophage polarization by sponging miR-512-3p to increase NOVA2, while Gentiana macrophylla flavonoids suppress NSCLC progression partly by downregulating circ_0059665. |
| Confidence score: |
0.8175 |