circRNA basic information
circBase ID: hsa_circ_0001955
Name: -
Synonym: circular RNA 0001955
Host Gene: -
Genomic location(hg19): chr15:64495280-64508912:-
Genomic location(hg38): chr15:64203081-64216713:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; para-cancer tissues; paracancerous tissues

Cell lines:

BEAS-2B; H520; A549; H358; H460; HCC827; HEK-293 T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: EGFR
Associated microRNA: miR-769-5p
Biological function: promotes NSCLC cell proliferation, migration and cell cycle progression; inhibits apoptosis; promotes NSCLC progression
Molecular mechanism: circ_0001955 acts as a ceRNA by adsorbing miR-769-5p, thereby upregulating EGFR expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes); EGFR (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot

Clinical significance:

high circ_0001955 expression was associated with advanced TNM stage, lymph node metastasis, unfavorable pathological characteristics, and worse prognosis in NSCLC patients

Description:

circ_0001955 is up-regulated in NSCLC tissues and cell lines and is associated with advanced TNM stage and lymph node metastasis. Functionally, circ_0001955 promotes proliferation, migration and cell cycle progression while inhibiting apoptosis in NSCLC cells. Mechanistically, cytoplasmic circ_0001955 acts as a ceRNA that sponges miR-769-5p and indirectly upregulates EGFR expression to facilitate NSCLC progression.

Confidence score:

0.73

Other information
Title:

Circ_0001955 promotes the progression of non-small cell lung cancer via miR-769-5p/EGFR axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 35920610
Study type:

combined biological and clinical study

Data availability: GSE101684
Code availability: -