| Expression pattern: |
UN |
| Associated gene: |
Caspase3, Caspase7, Caspase9, E-cadherin, Vimentin, N-cadherin |
| Associated microRNA: |
hsa-miR-181b-3p, hsa-miR-337-3p, hsa-miR-421, hsa-miR-548d-3p |
| Biological function: |
Oe-circ_0067582 inhibits gastric cancer cell viability, proliferation, invasion and tumor growth, induces apoptosis, and attenuates EMT. |
| Molecular mechanism: |
hsa_circ_0067582 overexpression regulates apoptosis-related proteins and EMT markers; bioinformatics prediction suggests a ceRNA mechanism through hsa-miR-181b-3p, hsa-miR-337-3p, hsa-miR-421 and hsa-miR-548d-3p with downstream cancer-related pathways including TGF-beta and p53 signaling. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); apoptosis (promotes); EMT (inhibits); TGF-beta/SMAD (other); p53 signaling (other); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
provides a new target for the treatment of GC |
| Description: |
This study investigated hsa_circ_0067582 in gastric cancer cell models and found that its overexpression suppresses AGS and SGC-7901 cell proliferation and invasion while promoting apoptosis. In vivo, Oe-circ_0067582 inhibited SGC-7901 xenograft tumor growth. Mechanistically, the study suggests modulation of apoptosis and EMT markers and predicts a hsa_circ_0067582-mediated ceRNA network involving hsa-miR-181b-3p, hsa-miR-337-3p, hsa-miR-421 and hsa-miR-548d-3p. |
| Confidence score: |
0.4648 |