circRNA basic information
circBase ID: hsa_circ_0053277
Name: hsa_circ_NRBP1
Synonym: circ_0053277
Host Gene: NRBP1
Genomic location(hg19): chr2:27663993-27665124:+
Genomic location(hg38): chr2:27441126-27442257:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; normal tissues; CRC tumor tissues; corresponding nontumor tissues
Cell lines:

HCT116; SW480; SW620; HT29; RKO; LOVO; HCoEpiC
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MMP14, Ago2
Associated microRNA: miR-2467-3p
Biological function: Promotes CRC cell proliferation, migration, and EMT process; knockdown suppresses these malignant phenotypes.
Molecular mechanism: hsa_circ_0053277 acts as a sponge for miR-2467-3p and upregulates MMP14 expression, thereby facilitating CRC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); EMT (promotes); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis
Clinical significance:

hsa_circ_0053277 could be treated as a therapeutic target for CRC patients.
Description:

hsa_circ_0053277 is up-regulated in colorectal cancer tissues and cells. It promotes CRC cell proliferation, migration, and EMT by acting as a miR-2467-3p sponge to increase MMP14 expression, suggesting potential therapeutic relevance.
Confidence score:

0.8203
Other information
Title:

Circular RNA hsa_circ_0053277 promotes the development of colorectal cancer by upregulating matrix metallopeptidase 14 via miR-2467-3p sequestration.

Journal: Journal of cellular physiology
Published: 2020
PubMed ID: 31549406
Study type:

combined biological and clinical study
Data availability: Research data are not shared.
Code availability: -