| Expression pattern: |
UP |
| Associated gene: |
BIRC5 |
| Associated microRNA: |
miR-1182 |
| Biological function: |
promotes NSCLC cell proliferation and inhibits apoptosis; promotes xenograft tumor growth |
| Molecular mechanism: |
circCAMSAP1 acts as a miRNA sponge for miR-1182 to increase BIRC5 (survivin) expression; rescue experiments with BIRC5 lacking 3'UTR support the axis |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; TUNEL; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
potential diagnosis and therapeutic target for NSCLC |
| Description: |
circCAMSAP1 is up-regulated in NSCLC tissues and cell lines and promotes tumor cell proliferation while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-1182 to increase BIRC5 (survivin) expression, and BIRC5 rescue experiments support the circCAMSAP1/miR-1182/BIRC5 axis in NSCLC progression. |
| Confidence score: |
0.78 |