| Expression pattern: |
UN |
| Associated gene: |
REGgamma, REGgamma mRNA |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
Knock-down of CircRNA CDR1as enhanced the cytotoxic effects of low-dose Diosbulbin-B on gastric cancer cells, inhibited GC cell proliferation, and promoted GC cell apoptosis, while having little effect on hepatocytes. |
| Molecular mechanism: |
CircRNA CDR1as acts through a ceRNA mechanism by sponging miR-7-5p to increase REGgamma expression; knock-down of CircRNA CDR1as enhances low-dose DB-induced GC cell death via the miR-7-5p/REGgamma axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); chemoresistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
Targeting CircRNA CDR1as may enhance the therapeutic efficiency of low-dose Diosbulbin-B for gastric cancer treatment. |
| Description: |
This in vitro study shows that CircRNA CDR1as supports gastric cancer cell survival under low-dose Diosbulbin-B treatment. Knock-down of CircRNA CDR1as enhances low-dose DB-induced inhibition of proliferation and induction of apoptosis in GC cells through the miR-7-5p/REGgamma axis, suggesting CDR1as as a potential target to improve DB-based GC therapy. |
| Confidence score: |
0.5607 |