circRNA basic information
circBase ID: hsa_circ_0001346
Name: hsa_circ_RNF13
Synonym: circRNF13
Host Gene: RNF13
Genomic location(hg19): chr3:149563797-149639014:+
Genomic location(hg38): chr3:149846010-149921227:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung adenocarcinoma samples; paired peripheral normal lung tissues; cancer tissues; LAC tissues
Cell lines:

A549; H1299; H2228; pc9; H1975; H827; SPCA-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: Ago2
Associated microRNA: miR-93-5p
Biological function: circRNF13 represses migration, invasion and metastasis of lung adenocarcinoma cells; knockdown promotes migration and invasion, while overexpression inhibits migration and invasion.
Molecular mechanism: circRNF13 acts as a cytoplasmic ceRNA/miRNA sponge for miR-93-5p through association with Ago2, suggesting post-transcriptional regulation.
Biological pathway or process:

migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; Bioinformatics Analysis
Clinical significance:

circRNF13 expression is significantly negatively correlated with TNM staging and lymph node metastasis, and circRNF13 represents a potential novel biomarker and therapeutic target of lung adenocarcinoma.
Description:

circRNF13 (hsa_circ_0001346), derived from RNF13, is down-regulated in lung adenocarcinoma tissues and is associated with TNM stage and lymph node metastasis. It mainly localizes in the cytoplasm and suppresses LAC cell migration, invasion and metastasis, at least partly by acting as an Ago2-associated sponge for miR-93-5p. The study proposes circRNF13 as a potential biomarker and therapeutic target for lung adenocarcinoma.
Confidence score:

0.7974
Other information
Title:

CircRNF13 regulates the invasion and metastasis in lung adenocarcinoma by targeting miR-93-5p.

Journal: Gene
Published: 2018
PubMed ID: 29704631
Study type:

combined biological and clinical study
Data availability: circBase
Code availability: -