circRNA basic information
circBase ID: hsa_circ_0074027
Name: hsa_circ_PITX1
Synonym: circ-PITX1 / circular RNA PITX1
Host Gene: PITX1
Genomic location(hg19): chr5:134363423-134369964:-
Genomic location(hg38): chr5:135027733-135034274:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBM tissues; normal tissues
Cell lines:

A172; LN229; U251; U87; NHA
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MAP3K2, RISC
Associated microRNA: miR-379-5p
Biological function: Promotes GBM cell proliferation and suppresses apoptosis; knockdown of circ-PITX1 suppresses proliferation and promotes apoptosis of GBM cells.
Molecular mechanism: circ-PITX1 acts as a ceRNA by sponging miR-379-5p to elevate MAP3K2 expression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circ-PITX1 is upregulated in glioblastoma tissues and cell lines. It promotes GBM cell proliferation and inhibits apoptosis by acting as a cytoplasmic ceRNA that sponges miR-379-5p and increases MAP3K2 expression.
Confidence score:

0.8195
Other information
Title:

Circular RNA circ-PITX1 promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-379-5p/MAP3K2 axis.

Journal: European journal of pharmacology
Published: 2019
PubMed ID: 31493405
Study type:

combined biological and clinical study
Data availability: Supplementary data; https://doi.org/10.1016/j.ejphar.2019.172643
Code availability: -