circRNA basic information
circBase ID: hsa_circ_0066903
Name: hsa_circ_GSK3B
Synonym: circ-GSK3B
Host Gene: GSK3B
Genomic location(hg19): chr3:119666114-119721086:-
Genomic location(hg38): chr3:119947267-120002239:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LUAD tissue samples; adjacent normal tissue samples; tumor tissues; lungs
Cell lines:

BEAS-2B; PC9; H2073; H-1975; A549
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: GSK3B, FKBP51, GSK-3beta
Associated microRNA: miR-3681-3p, miR-3909
Biological function: Suppresses LUAD cell proliferation, migration, EMT, stemness, tumor growth and metastasis.
Molecular mechanism: circ-GSK3B acts as a ceRNA sponging miR-3681-3p and miR-3909 to up-regulate GSK3B; it also binds FKBP51 and impedes FKBP51 binding to GSK-3beta, reducing GSK-3beta S9 phosphorylation and blocking Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); EMT (inhibits); stemness (inhibits); metastasis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

Circ-GSK3B may act as an anti-tumor gene and potential therapeutic target in LUAD development.
Description:

circ-GSK3B (hsa_circ_0066903) is down-regulated in LUAD tissues and cells and functions as a tumor suppressor. It sponges miR-3681-3p and miR-3909 to increase GSK3B expression, and also binds FKBP51 to prevent FKBP51-GSK-3beta interaction, thereby inhibiting GSK-3beta S9 phosphorylation and inactivating Wnt/beta-catenin signaling. Functionally, circ-GSK3B suppresses LUAD proliferation, migration, EMT, stemness, tumor growth, and metastasis.
Confidence score:

0.8711
Other information
Title:

Circ-GSK3B up-regulates GSK3B to suppress the progression of lung adenocarcinoma.

Journal: Cancer gene therapy
Published: 2022
PubMed ID: 35821283
Study type:

combined biological and clinical study
Data availability: All data available on reasonable request from the corresponding author, MZ; Supplementary material available at https://doi.org/10.1038/s41417-022-00489-8
Code availability: -