| Expression pattern: |
UP |
| Associated gene: |
SPP1, AGO2, QKI |
| Associated microRNA: |
miR-200a-3p, miR-141-3p |
| Biological function: |
promotes pre-metastatic niche formation and colorectal cancer liver metastasis by activating SPP1highCD206+ pro-tumor macrophages and promoting CRC cell migration |
| Molecular mechanism: |
TEVs-released circ-0034880 acts as a miRNA sponge for miR-200a-3p and miR-141-3p to protect SPP1 from miRNA-mediated degradation, increasing SPP1 and activating SPP1highCD206+ pro-tumor macrophages; circ-0034880 biogenesis is promoted by QKI and can be reduced by Ginsenoside Rb1 via binding QKI |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
M
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RNA-seq; Microarray; Clinical Sample Validation; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; ELISA; Western Blot; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circ-0034880 is highly enriched in plasma EVs from CRC patients and is higher in CRC patients with liver metastases, suggesting association with CRLM and potential diagnostic marker |
| Description: |
circ-0034880 is up-regulated/enriched in CRC patient plasma EVs and is further increased in CRC with liver metastasis. Tumor-derived EVs deliver circ-0034880 to liver macrophages, where it sponges miR-200a-3p and miR-141-3p to increase SPP1, promoting SPP1highCD206+ pro-tumor macrophage activation, PMN formation, and liver metastasis. Ginsenoside Rb1 inhibits circ-0034880 biogenesis via targeting the RBP QKI, reducing downstream macrophage activation and metastasis. |
| Confidence score: |
0.7471 |