circRNA basic information
circBase ID: -
Name: hsa_circ_BNC2
Synonym: circBNC2
Host Gene: BNC2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0000494
MONDO name: renal fibrosis
Disease details: kidney fibrosis / renal fibrosis
Disease DO ID:
0050855
Disease MeSH ID:
-
Disease NCIt ID:
-
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

renal tubules; renal cortex; kidney tissue; liver tissue; renal biopsy specimens; liver biopsies

Cell lines:

HK2; HKC8; L-02; HEK-293T; LX-2; sv40mes13; mTEC; MPC-5

In vivo animal model:

other disease animal model

circRNA-disease information
Expression pattern:
DN
Associated gene: DHX9, ADAR1, CDK1, cyclin B1
Associated microRNA: -
Biological function: inhibits epithelial cell G2/M arrest, reduces profibrotic factor secretion, and mitigates extracellular matrix deposition and fibrosis in kidney and liver injury models
Molecular mechanism: circBNC2 encodes a translated protein (ctBNC2) that promotes CDK1/cyclin B1 complex formation and nuclear translocation; circBNC2 downregulation after injury is partially mediated by DHX9 upregulation (with ADAR1 facilitating DHX9 binding)
Biological pathway or process:

cell cycle (inhibits); proliferation (promotes); fibrosis (inhibits)

Detected method:
Q
B
H
S
Validation methods:

RNA-seq; RT-qPCR; divergent primers PCR; Sanger Sequencing; Northern Blot; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); TUNEL; ELISA; Cell Cycle Assay

Clinical significance:

Downregulation of circBNC2 is recapitulated in human IRI-induced chronic kidney disease and HBV-induced liver fibrosis, supporting clinical relevance as a potential therapeutic intervention target.

Description:

circBNC2 is downregulated in kidney and liver fibrotic injury settings. It suppresses epithelial G2/M arrest and profibrotic factor secretion by encoding ctBNC2, which promotes CDK1/cyclin B1 complex formation and supports mitotic entry, thereby mitigating maladaptive repair and fibrosis.

Confidence score:

0.8741

Other information
Title:

Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair.

Journal: Nature communications
Published: 2022
PubMed ID: 36316334
Study type:

combined biological and clinical study

Data availability: GSE179620; GSE179506; GSE179505; PXD033284
Code availability: -