| Expression pattern: |
UP |
| Associated gene: |
MEX3A, MOV10, PABP1, UPF1, PDE5A |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation and migration, promotes tumor growth, suppresses autophagy, and facilitates PBs aggregation leading to mRNA decay |
| Molecular mechanism: |
circMPP6 acts as a scaffold for MEX3A and PBs proteins (MOV10, PABP1, UPF1), promoting PBs aggregation and UPF1-mediated PDE5A mRNA decay, thereby inhibiting autophagy and promoting CRC aggressiveness |
| Biological pathway or process: |
autophagy (inhibits); proliferation (promotes); migration (promotes); ceRNA regulation (other); mRNA stability (other) |
| Detected method: |
Q
M
|
| Validation methods: |
divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Microarray |
| Clinical significance: |
circMPP6 is upregulated in CRC tissues and is negatively correlated with PDE5A expression in CRC tissues (TCGA and SYSUCC cohorts) |
| Description: |
circMPP6 is upregulated in colorectal cancer and predominantly localized in the cytoplasm. It serves as a scaffold to strengthen MEX3A association with PBs components (e.g., MOV10, PABP1, UPF1), promoting PBs aggregation and UPF1-mediated PDE5A mRNA decay, which suppresses autophagy and enhances CRC proliferative/migratory and tumorigenic properties. |
| Confidence score: |
0.8439 |