| Expression pattern: |
UP |
| Associated gene: |
ILK, NF-kappaB p65, NF-kappaB p65 (Ser536) |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration, invasion and tumor growth of gastric cancer cells; protects ILK protein from degradation; activates NF-kappaB signaling; high expression is associated with poor overall survival. |
| Molecular mechanism: |
circ_PABPC1 directly binds ILK protein, protects ILK from degradation, promotes ILK-NF-kappaB p65 interaction and phosphorylation of NF-kappaB p65, thereby activating the NF-kappaB signaling pathway. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; MTT; EdU Staining; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_PABPC1 expression is associated with poor prognosis and poorer overall survival in gastric cancer; circ_PABPC1 has potential as a biomarker of GC. |
| Description: |
circ_PABPC1/hsa_circ_0008496 is up-regulated in gastric cancer tissues and cell lines, and high expression correlates with poor overall survival. Functionally, it promotes proliferation, migration, invasion and xenograft tumor growth of GC cells. Mechanistically, circ_PABPC1 interacts with ILK protein, protects ILK from degradation, and activates NF-kappaB signaling. |
| Confidence score: |
0.8891 |