| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
circBCL11B supports leukemic cell proliferation and survival under stress conditions; knockdown reduced proliferation and increased cell death/apoptosis in AML cell lines. |
| Molecular mechanism: |
circBCL11B is formed by circularization of exon 2 of BCL11B and is associated with a T-cell-like gene expression signature in AML; its precise molecular mechanism was not defined in this study. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNA-seq; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
circBCL11B was exclusively expressed in AML patients compared with healthy HSPCs and was associated with a T-cell-like gene expression signature; the association was validated in an independent AML cohort. |
| Description: |
circBCL11B is a BCL11B exon 2-derived circular RNA that was detected exclusively in AML samples and not in healthy HSPCs. Its high expression was associated with a T-cell-like gene expression signature, and shRNA-mediated knockdown impaired AML cell proliferation and increased cell death/apoptosis under serum-deprivation stress. The study supports circBCL11B as a functionally relevant AML-associated circRNA candidate, although its detailed molecular mechanism remains unresolved. |
| Confidence score: |
0.6369 |