| Expression pattern: |
UP |
| Associated gene: |
DNMT3A, BCL6B, BCL6B promoter, AGO2 |
| Associated microRNA: |
miR-29c-3p |
| Biological function: |
circTFF1 promotes lung cancer cell proliferation, migration and invasion. |
| Molecular mechanism: |
circTFF1 functions as a ceRNA by sponging miR-29c-3p to upregulate DNMT3A, which facilitates methylation of the BCL6B promoter and represses BCL6B expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; MSP (Methylation-Specific PCR); Western Blot; ChIP / ChIP-seq; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circTFF1 is upregulated in lung cancer cell lines and promotes malignant phenotypes including proliferation, migration and invasion. Mechanistically, circTFF1 sponges miR-29c-3p to increase DNMT3A expression, thereby facilitating BCL6B promoter methylation and suppressing the tumor-suppressive role of BCL6B. |
| Confidence score: |
0.7027 |