| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, CCAR1 mRNA, ubiquitin-proteasome pathway |
| Associated microRNA: |
- |
| Biological function: |
inhibits trophoblast cell proliferation; inhibits trophoblast cell migration; inhibits trophoblast cell invasion |
| Molecular mechanism: |
circFTO directly binds and negatively regulates IGF2BP2, promotes IGF2BP2 ubiquitination and proteasomal degradation, and alters m6A modification and stability of CCAR1 mRNA via the m6A reader IGF2BP2 |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); m6A modification (other); mRNA stability (other); ubiquitination (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; RIP (RNA Immunoprecipitation); Western Blot; Co-IP; Actinomycin D / DRB Stability Assay; MeRIP / MeRIP-seq; Bioinformatics Analysis; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circFTO dysregulation may be implicated in the occurrence of SA, highlighting its potential as a biomarker for SA. |
| Description: |
circFTO (hsa_circ_0005941) is up-regulated in placental villi from SA patients and suppresses trophoblast proliferation, migration, and invasion. Mechanistically, circFTO binds IGF2BP2 and promotes its ubiquitination/proteasomal degradation, thereby altering IGF2BP2-dependent m6A recognition and the stability/m6A modification of CCAR1 mRNA in trophoblasts. |
| Confidence score: |
0.8314 |