| Expression pattern: |
UP |
| Associated gene: |
AKT1 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
circ_0067997 promotes gastric cancer cell growth, tumor formation, DDP resistance, and inhibits apoptosis; circ_0067997 knockdown restricts SGC-7901/DDP cell proliferation and tumor-inducing ability and enhances apoptosis in the presence of DDP. |
| Molecular mechanism: |
circ_0067997 acts as a sponge for miR-615-5p and regulates AKT1 expression through a circ_0067997/miR-615-5p/AKT1 axis, thereby promoting growth and inhibiting apoptosis of DDP-insensitive gastric cancer cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); chemoresistance (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; TUNEL |
| Clinical significance: |
circ_0067997 expression was linked to GC severity, histological type, clinical stage and lymph node metastasis, and was proposed as a useful molecule and therapeutic target for detection and management of GC. |
| Description: |
circ_0067997 is up-regulated in gastric cancer, especially in cisplatin-insensitive GC tissues and SGC-7901/DDP cells. It promotes GC cell growth, tumor formation and DDP resistance while inhibiting apoptosis, at least partly by sponging miR-615-5p to regulate AKT1. Its expression is associated with clinicopathologic severity and may represent a detection and therapeutic target for GC. |
| Confidence score: |
0.7032 |