| Expression pattern: |
UP |
| Associated gene: |
HMGB2, AGO2 |
| Associated microRNA: |
miR-1225-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, sphere formation and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0075048 acts as a miR-1225-5p sponge to up-regulate HMGB2 expression in NSCLC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); stemness (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; Cohort Study; Survival Analysis |
| Clinical significance: |
High circ_0075048 expression was associated with poorer overall survival, advanced TNM stages and tumor size in NSCLC patients. |
| Description: |
hsa_circ_0075048 is up-regulated in NSCLC tissues and cell lines and is associated with poor overall survival, advanced TNM stage and tumor size. Functionally, it promotes malignant phenotypes including proliferation, migration, invasion, sphere formation and in vivo tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic circ_0075048 sponges miR-1225-5p to increase HMGB2 expression. |
| Confidence score: |
0.8823 |