circRNA basic information
circBase ID: hsa_circ_0100181
Name: hsa_circ_PAN3
Synonym: circPAN3 / circRNA_100181
Host Gene: PAN3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018874
MONDO name: acute myeloid leukemia
Disease details: acute myeloid leukemia / AML
Disease DO ID:
 9119
Disease MeSH ID:
 D015470
Disease NCIt ID:
 C3171
Disease ICD11 ID:
 -
Disease OMIM ID:
 601626
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow / BM samples / BM tissue specimens
Cell lines:

THP-1; K562; THP-1/ADM; K562/ADM
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TAK1, AMPK, mTOR, LC3-I, LC3-II, Beclin-1, P62, Bax, Bcl-2, Caspase-3, Caspase-9
Associated microRNA: miR-153-5p, miR-183-5p, miR-338-3p, miR-346, miR-545-3p, miR-574-5p, miR-599, miR-653-5p, miR-766-3p, miR-767-3p
Biological function: circPAN3 facilitates ADM resistance in AML cells by enhancing autophagy activity and reducing ADM-induced apoptosis; circPAN3 knockdown restores drug sensitivity, while circPAN3 overexpression decreases ADM-induced apoptosis.
Molecular mechanism: circPAN3 acts as an autophagy inducer in AML drug resistance through AMPK/mTOR signaling; it may function as a miRNA sponge, including the circPAN3-miR-545-3p-TAK1 axis, to activate AMPK/mTOR signaling and regulate autophagy.
Biological pathway or process:

autophagy (promotes); AMPK (promotes); PI3K/AKT/mTOR (other); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Western Blot; Bioinformatics Analysis
Clinical significance:

circPAN3 was increased in BM samples from relapsed AML patients and may be a potential therapeutic target for drug-resistance AML.
Description:

circPAN3 is up-regulated in ADM-resistant AML cell lines and bone marrow samples from relapsed AML patients. It promotes AML drug resistance by enhancing autophagy and suppressing ADM-induced apoptosis, largely through activation of AMPK/mTOR signaling. The study also suggests a possible ceRNA mechanism involving circPAN3-miR-545-3p-TAK1, and proposes circPAN3 as a potential therapeutic target for drug-resistant AML.
Confidence score:

0.7291
Other information
Title:

CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy.

Journal: Leukemia research
Published: 2019
PubMed ID: 31401408
Study type:

combined biological and clinical study
Data availability: Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.leukres.2019.106198.
Code availability: STAR: https://github.com/alexdobin/STAR; DCC: https://github.com/dieterich-lab/DCC/tree/master/DCC; Bioconductor: https://www.bioconductor.org/