| Expression pattern: |
DN |
| Associated gene: |
PTEN, PI3K, AKT, SP-1, CyclinD1, cleaved-Caspase-3, cleaved-Caspase-9 |
| Associated microRNA: |
miR-22 |
| Biological function: |
Overexpression of circ-ITCH facilitated apoptosis and repressed viability, proliferation, migration and invasion in osteosarcoma MG63 and Saos-2 cells, indicating a tumour suppressor role. |
| Molecular mechanism: |
circ-ITCH down-regulates miR-22 and suppresses PTEN/PI3K/AKT and SP-1 pathways in osteosarcoma cells. |
| Biological pathway or process: |
PI3K/AKT (inhibits); apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot |
| Clinical significance: |
circ-ITCH may act as a biomarker and regulator and provided an innovative target for clinical treatment in OS progression. |
| Description: |
circ-ITCH is down-regulated in osteosarcoma tissues and cell lines. Functionally, circ-ITCH overexpression suppresses osteosarcoma cell viability, proliferation, migration and invasion while promoting apoptosis. Mechanistically, the study reports that circ-ITCH acts through down-regulation of miR-22 and inhibition of PTEN/PI3K/AKT and SP-1 pathways, suggesting a tumour-suppressive role and potential biomarker or therapeutic target value. |
| Confidence score: |
0.5434 |