| Expression pattern: |
UP |
| Associated gene: |
SPAG6, EIF4A3, PTEN, PI3K, AKT |
| Associated microRNA: |
- |
| Biological function: |
Promotes BC cell proliferation, migration, and invasion; facilitates tumor growth in vivo. |
| Molecular mechanism: |
Recruits EIF4A3 to increase SPAG6 mRNA stability and expression; modulates PTEN/PI3K/AKT signaling via SPAG6. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circMYH9 expression was associated with poor prognosis in BC patients. |
| Description: |
CircMYH9 is up-regulated in breast cancer and promotes malignant phenotypes (proliferation, migration, invasion) and tumor growth. It recruits the RBP EIF4A3 to enhance SPAG6 mRNA stability/expression, which in turn suppresses PTEN and activates the PI3K/AKT pathway, associating high circMYH9 with poor prognosis. |
| Confidence score: |
0.7892 |