circRNA basic information
circBase ID: hsa_circ_0010889
Name: hsa_circ_PITHD1
Synonym: circ-PITHD1
Host Gene: PITHD1
Genomic location(hg19): chr1:24104875-24114722:+
Genomic location(hg38): chr1:23778385-23788232:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: Colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent normal tissues; CRC tumor tissues
Cell lines:

SW480; SW620; HT29; HCT116; Lovo; NCM460; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HK2
Associated microRNA: miR-590-5p
Biological function: promotes CRC proliferation, migration/invasion, tumor growth and pulmonary metastasis; enhances aerobic glycolysis
Molecular mechanism: ceRNA mechanism: circ-PITHD1 sponges miR-590-5p to upregulate HK2, thereby promoting glycolysis (Warburg effect)
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

circ-PITHD1 may represent a novel CRC diagnosis and treatment target; promising biomarker in CRC diagnostics
Description:

circ-PITHD1 is up-regulated in colorectal cancer tissues and cell lines and promotes proliferation, migration/invasion, tumor growth and lung metastasis. Mechanistically, it acts as a ceRNA by sponging miR-590-5p to increase HK2 expression, thereby enhancing aerobic glycolysis (Warburg effect).
Confidence score:

0.7486
Other information
Title:

Downregulation of Circ-PITHD1 Suppressed Colorectal Cancer via Glycolysis Inhibition through miR-590-5p/HK2 Axis.

Journal: Evidence-based complementary and alternative medicine : eCAM
Published: 2022
PubMed ID: 36276867
Study type:

combined biological and clinical study
Data availability: All data in this study are available from the corresponding author.
Code availability: -