circRNA basic information
circBase ID: -
Name: hsa_circ_ZFR
Synonym: hsa_circRNA_103809
Host Gene: ZFR
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paired normal adjacent tissues; xenograft tumor tissues
Cell lines:

MHCC97L; Huh7; SK-HEP-1; Hep3B; HCCLM3; LO2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FGFR1, Ago2, ERK1/2, p-ERK1/2, Cyclin D1, CDK4, CDK6, E-cadherin, N-cadherin
Associated microRNA: miR-377-3p
Biological function: Promotes HCC cell proliferation, cell cycle progression, migration, invasion, and xenograft tumor growth; knockdown suppresses these malignant phenotypes.
Molecular mechanism: hsa_circRNA_103809 acts through a ceRNA-like mechanism by binding miR-377-3p, negatively regulating miR-377-3p, increasing FGFR1 expression, and activating ERK-related signaling.
Biological pathway or process:

ERK (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other)
Detected method:
Q
H
M
Validation methods:

RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circRNA_103809 expression is associated with lower overall survival and adverse clinicopathological features including vascular invasion, cirrhosis, tumor size, and Edmindson grade.
Description:

hsa_circRNA_103809 is up-regulated in HCC tissues and cell lines, and high expression predicts poorer survival. Functionally, it promotes HCC proliferation, cell cycle progression, migration, invasion, and xenograft tumor growth. Mechanistically, it binds and suppresses miR-377-3p, thereby increasing FGFR1 expression and affecting the FGFR1/ERK-related oncogenic program.
Confidence score:

0.8457
Other information
Title:

Circular RNA hsa_circRNA_103809 promoted hepatocellular carcinoma development by regulating miR-377-3p/FGFR1/ERK axis.

Journal: Journal of cellular physiology
Published: 2020
PubMed ID: 31317555
Study type:

combined biological and clinical study
Data availability: GSE97332
Code availability: -