| Expression pattern: |
UP |
| Associated gene: |
B4GALT1, p65 |
| Associated microRNA: |
miR-124 |
| Biological function: |
circHIPK3 promotes CML cell proliferation, inhibits apoptosis, and is associated with poor prognosis; knockdown of circHIPK3 inhibits K562 and Kasumi4 cell proliferation and induces apoptosis. |
| Molecular mechanism: |
circHIPK3 may function through a circHIPK3/miR-124 axis, with miR-124 negatively correlated with circHIPK3 and B4GALT1 and p65 identified as direct targets of miR-124. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Cohort Study; Survival Analysis |
| Clinical significance: |
High circHIPK3 expression predicted poor outcome and shorter overall survival in CML patients; serum circHIPK3 level was an independent prognostic factor. |
| Description: |
circHIPK3 is upregulated in PBMC and serum samples from CML patients and high serum circHIPK3 expression predicts poor overall survival. Functionally, circHIPK3 acts as an oncogenic circRNA in CML by promoting proliferation and suppressing apoptosis of CML cells. Mechanistically, the study suggests a circHIPK3/miR-124 axis involving downstream miR-124 targets such as B4GALT1 and p65. |
| Confidence score: |
0.7169 |