| Expression pattern: |
UP |
| Associated gene: |
Wnt7B, SP1, EPHB4, beta-catenin, AXIN2, c-myc, AGO2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes glioblastoma cell growth, proliferation, cell-cycle progression, invasion, EMT phenotype, tumorigenesis in vivo, and activates the Wnt/beta-catenin pathway. |
| Molecular mechanism: |
SP1 increases circ 0001730 expression through the EPHB4 promoter; cytoplasmic circ 0001730 acts as a ceRNA/miR-326 sponge, increasing Wnt7B expression and activating the Wnt/beta-catenin pathway. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); invasion (promotes); migration (promotes); EMT (promotes); metastasis (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ 0001730 expression was associated with clinical stage; high circ 0001730 expression predicted poorer overall survival and progression-free survival in high-grade glioma patients; circ 0001730 could be a promising therapeutic target for glioblastoma treatment. |
| Description: |
circ 0001730 is upregulated in glioblastoma/glioma tissues and cell lines and its high expression is associated with advanced clinical stage and poorer survival. Functionally, circ 0001730 promotes glioblastoma cell proliferation, cell-cycle progression, invasion, EMT and in vivo tumor growth. Mechanistically, SP1 upregulates circ 0001730 via the host gene EPHB4, and cytoplasmic circ 0001730 sponges miR-326 to increase Wnt7B and activate Wnt/beta-catenin signaling. |
| Confidence score: |
0.8973 |