| Expression pattern: |
UP |
| Associated gene: |
G3BP2 |
| Associated microRNA: |
miR-556-3p |
| Biological function: |
accelerates CRC cell proliferation, migration and stemness; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: hsa_circRNA_001676 sponges miR-556-3p to increase G3BP2, associated with increased stemness markers (Oct-4, Nanog) |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; TUNEL; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; H&E Staining |
| Clinical significance: |
potential therapeutic target in CRC |
| Description: |
hsa_circRNA_001676 is up-regulated in colorectal cancer tissues and CRC cell lines and promotes proliferation, migration, and stemness. It functions as a ceRNA by sponging miR-556-3p, thereby increasing G3BP2 expression and enhancing stemness-associated markers; its knockdown suppresses tumor growth in a CRC xenograft model. |
| Confidence score: |
0.7376 |