| Expression pattern: |
UP |
| Associated gene: |
HPSE / heparanase |
| Associated microRNA: |
miR-184 |
| Biological function: |
cZNF609 may regulate inflammation-induced corneal lymphangiogenesis and lymphangiogenic mediator expression, potentially by acting as an endogenous miRNA sponge for miR-184. |
| Molecular mechanism: |
Potential ceRNA/miRNA sponge mechanism: cZNF609 may bind miR-184 and regulate HPSE expression by RNA silencing and post-transcriptional regulation. |
| Biological pathway or process: |
ceRNA regulation (other); angiogenesis (promotes); other pathway/process (promotes); inflammation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; IF (Immunofluorescence); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
cZNF609 was overexpressed in inflamed human corneas induced by chemical burns, suggesting clinical relevance to corneal inflammatory lymphangiogenesis. |
| Description: |
This study identified cZNF609 as an up-regulated circRNA in inflammation-induced lymphangiogenesis using microarray profiling and validated its elevation by qRT-PCR in inflamed HLECs, mouse corneal LG models, and human inflamed corneas. cZNF609 was mainly cytoplasmic and RNase R-resistant, and the authors proposed that it may act as a miR-184 sponge to regulate HPSE expression and lymphangiogenic processes, although the direct sponging mechanism requires further verification. |
| Confidence score: |
0.7091 |