| Expression pattern: |
UP |
| Associated gene: |
FLI1, claudin-5, occludin, ZO-1, Ago2 |
| Associated microRNA: |
miR-194-5p |
| Biological function: |
Regulates BTB permeability and barrier integrity in glioma endothelial BTB model; knockdown increases permeability and decreases tight junction proteins (claudin-5, occludin, ZO-1); enhances doxorubicin delivery across BTB and increases glioma cell apoptosis when combined with miR-194-5p overexpression. |
| Molecular mechanism: |
circ-USP1 acts as a miRNA sponge binding miR-194-5p (Ago2-associated), suppressing miR-194-5p activity; miR-194-5p targets and down-regulates transcription factor FLI1, which transcriptionally activates claudin-5, occludin and ZO-1 to regulate BTB permeability. |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Transfection; CCK8; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
Potential therapeutic target to facilitate drug delivery across BTB in glioma. |
| Description: |
circ-USP1 is up-regulated in glioma-associated cerebral microvascular endothelial cells in an in vitro BTB model. It binds and suppresses miR-194-5p, thereby maintaining FLI1 expression and tight junction proteins (claudin-5, occludin, ZO-1) to reduce BTB permeability; circ-USP1 knockdown increases permeability and can enhance doxorubicin-induced glioma cell apoptosis across the BTB. |
| Confidence score: |
0.6959 |